牦牛KDM1A基因克隆及其在不同发育时期睾丸中的表达规律

doi:10.11843/j.issn.0366-6964.2018.12.028

Abstract

Abstract:

The aim of this study was to clone the lysine-specific histone demethylase 1A (KDM1A) gene, identify its expression in various tissues of yak, and to analyze the expression pattern in different growth periods of yak testis. The total RNA was extracted by collecting the heart, spleen, liver, ovary, lung, cerebrum, kidney, uterus, large intestine, testis and stomach from healthy yaks aged 4-5 years old.In addition,the testes at different developmental stages were collected including fetus (5-6-month-old), infancy (1-2-year-old), sexual maturity (4-5-year-old) and old age (9-10-year-old).RT-PCR was adopted for amplification of the complete CDS of KDM1A gene in yak.Meanwhile the structure and function of KDM1A gene in yak were analyzed by a series of bioinformatics softwares.Then the expression of KDM1A in different tissues and testes at different developmental stages were detected by RT-qPCR.The results showed that the KDM1A was obtained by cDNA cloning,the length of which was 2 401 bp.And the length of the KDM1A gene was 2 331 bp in CDS, encoding 776 amino acids.It was high homology identity to that of corresponding cDNA from bovine,which showed that the KDM1A gene was conservative in the process of evolution.The expression profile of KDM1A was wide in yak tissues,but there are some differences in various tissues.For example,the expression level was the highest in liver and testis.The expression level of KDM1A mRNA during testis development presented the tendency of going up firstly and going down secondly. The complete CDS of KDM1A gene was successfully cloned and the KDM1A had a significant different expression among the 4 periods of yak testis,which indicted that the KDM1A gene might play an important role in testis development of yak.

CLC Number:

S823.8⁺5.2

HAN Jie, XIONG Xian-rong, WANG Yan, YANG Xian-ying, A GUO Yue-da, HUANG Xiang-yue, LI Jian. Cloning of Yak KDM1A Gene and Its Expression Profile in Testis during Different Developmental Stages[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(12): 2777-2785.

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Cloning of Yak KDM1A Gene and Its Expression Profile in Testis during Different Developmental Stages

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